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发货规格 |
1×10⁶ |
别名 |
SAOS-2; Saos-2; SAOS 2; Saos 2; Saos2; SaOs2; SAOS2; Sarcoma OSteogenic-2; SaOS; SAOS |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
McCOY’s5A+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
48h |
传代比例 |
1:2~1:4 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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种属 |
人源 |
组织来源 |
骨 |
肿瘤类型 |
骨肉瘤 |
供体信息 |
11岁白人女性 |
背景描述 |
Saos-2 细胞是一种骨肉瘤细胞系,来源于 11 岁白种人女性的原发性成骨肉瘤。这些细胞是研究骨肉瘤和骨生物学的广泛认可模型,因为它们具有成骨细胞特征和产生骨样细胞外基质的能力。Saos-2 细胞具有高水平的碱性磷酸酶活性和骨骼特异性蛋白(如骨钙素和骨桥蛋白)的表达,可作为研究骨形成和骨肉瘤病理生理学的有效体外系统。它们对于研究细胞对模拟骨骼环境的各种生化刺激和机械力的反应特别有价值。 |
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受体表达 |
Epidermal growth factor (EGF), transforming growth factor beta (type 1 and type 2) |
同工酶 |
Me-2, 1, PGM3, 1-2, PGM1, 1-2, ES-D, 2, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0002 |
致瘤性 |
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
抗原表达 |
Blood Type B, Rh+, HLA A2, A3, Bw16, Bw47 |
逆转录酶 |
阴性 |
核型 |
The stemline chromosome number is hypotriploid with the modal number of 56 chromosomes per cell and the 2S component occurring at 13.2%. Over two-thirds of the chromosome complement consisted of structurally rearranged chromosomes. Most marker chromosomes had complex rearrangements. The origin of the segments composing these markers could not be identified. Of the identifiable markers, 6p+/q+, 7p+, 11p+, and 12p+ occasionally were present at 2 copies per cell. The Y chromosome was not detected in the QM stained preparation. |