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发货规格 |
1×10⁶ |
别名 |
U-2 OS; U-2OS; U-2-OS; U2-OS; U20-S; U20S; 2T |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
McCoy’s5A+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
25~30h |
传代比例 |
1:2~1:4 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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种属 |
人源 |
组织来源 |
胫骨 |
肿瘤类型 |
骨 肉瘤 |
供体信息 |
15岁白人女性 |
背景描述 |
U2OS 细胞是一种源自人类骨肉瘤患者的骨肉瘤细胞系,在癌症研究中发挥着重要作用,尤其是在骨癌研究中。U2OS 细胞广泛用于癌症研究、药物开发、细胞凋亡研究、遗传研究和放射肿瘤学研究。U2OS细胞的价值在于它们用于研究细胞凋亡和耐药性,这对于制造小分子抑制剂和类似治疗剂至关重要。 |
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蛋白表达 |
Osteosarcoma derived growth factor (ODGF) |
受体表达 |
Insulin-like growth factor I (IGF-I), insulin-like growth factor II (IGF-I), Osteosarcoma derived growth factor (ODGF) |
同工酶 |
PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0082 |
抗原表达 |
Blood Type A, Rh+, HLA A2, Aw30, B12, Bw35, B40(+/-) |
核型 |
Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range. We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified., Different chromosomal rearrangements involving the same chromosomes (N1, N7, N9, and N11 particularly), are seen. Twenty-two markers are found including: t(9qter--->9q21::1p36--->1p::?), 7p+, iso(17q), t(15q;?), 4q+, del(3)(q21), 5q(aberrant) and others. |