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| 发货规格 |
1×10⁶ |
| 别名 |
NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC); Clone 929; Earles’s cells; Earle’s L cells |
| 形态 |
成纤维细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
RPMI-1640+10%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
24~28h |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
小鼠 |
| 组织来源 |
正常皮下结缔组织 |
| 供体信息 |
100天雄性C3H/An小鼠 |
| 背景描述 |
L-929 细胞是一种成纤维细胞样细胞系，来源于 100 日龄雄性 C3H/An 小鼠的皮下结缔组织。该细胞系成立于 1940 年代，由于其稳健性、易于培养和应用多功能性，已成为各种生物和医学研究领域的关键。L-929 细胞的特征在于其纺锤形、成纤维细胞形态和贴壁生长。它们广泛用于细胞毒性测定，并作为评估材料生物相容性和各种物质毒性作用的标准模型，这在生物材料和组织工程领域尤为重要。 |
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| 致瘤性 |
Yes， in immunosuppressed mice |
| 抗原表达 |
H-2k |
| 病毒 |
Ectromelia virus (mousepox): negative |
| 病毒易感性 |
poliovirus 1， 2， 3， coxsackievirus B5， polyomavirus |
| 逆转录酶 |
阳性 |
| 核型 |
modal chromosome number = 66; range = 65 to 68. There were approximately 20 to 30 marker chromosomes present in each metaphase spread. A high percentage of those markers were common to most analyzed cells. A long metacentric chromosome with secondary constriction was noted in 77/100 cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
