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RAW264.7细胞(小鼠腹腔巨噬细胞细胞系)
RAW264.7细胞(小鼠腹腔巨噬细胞细胞系)
  • 商品货号:CL0266
  • 活动价: ¥899元
    市场价:¥1800元
    已售 57 件 | 评价 0 人次 | 关注度 14771
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    • 细胞信息
     
    发货规格 1×10⁶
    别名 RAW264; RAW2647; RAW264.7; RAW-264.7; Raw 264.7; Raw264.7
    形态 巨噬细胞样
    生长特性 贴壁
    培养条件 DMEM+10%FBS+1%P/S
    冻存条件 90%FBS+10%DMSO
    传代方式 刮刀消化
    倍增时间 11~30h
    传代比例 1:2~1:4
    换液频率 每周2~3次
    生物安全等级 1
    培养注意事项 使用刮刀消化
    • 细胞背景
     
    种属 小鼠
    组织来源 腹水
    肿瘤类型 白血病
    供体信息 成年雄性BALB/c小鼠
    背景描述 RAW 264.7 细胞是一种广泛使用的鼠巨噬细胞细胞系,来源于患有 Abelson 鼠白血病病毒诱导肿瘤的雄性小鼠腹水,通常用于免疫学和传染病研究。作为一种永生化细胞系,RAW264.7细胞是研究巨噬细胞生物学的关键模型系统,包括对病原体的免疫反应、信号转导和基因表达。
    • 遗传背景
     
    受体表达 Complement (C3)
    基因表达 lysozyme; H-2d; ectromelia virus (mousepox) negative.
    抗原表达 H-2d
    病毒 The cell line was tested and found positive for Reverse Transcriptase (RT) activity from C-Type retroviruses in cell culture supernatant and cell extract. Ectromelia virus (mousepox) may be secreted.
    STR鉴定
    STR位点信息
    STR鉴定图
    Amelogenin x,y
    M_18-3 18
    M_4-2 22.3,23.3
    M_6-7 12
    M_3-2 14
    M_19-2 12,14
    M_7-1 25.2
    M_1-1 15,16
    M_8-1 13
    M_2-1 16
    M_15-3 22.3
    M_6-4 18
    M_11-2 17
    M_1-2 17
    M_17-2 14,16
    M_12-1 16,17
    M_5-5 14
    M_X-1 25
    M_13-1 16.2
    HumanD4/D8 -

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