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U-118MG细胞(人脑星形胶质母细胞瘤)
U-118MG细胞(人脑星形胶质母细胞瘤)
  • 商品货号:CL0412
  • ¥1500元
    已售 19 件 | 评价 0 人次 | 关注度 1093
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    • 细胞信息
     
    发货规格 1×10⁶
    别名 U-118MG,U-118-MG,U118-MG,U118MG,u1118mg,118MG
    形态 混合细胞样
    生长特性 贴壁
    培养条件 DMEM+10%FBS+1%P/S
    冻存条件 90%FBS+10%DMSO
    传代方式 胰酶消化
    传代比例 1:3~1:6
    换液频率 每周2~3次
    生物安全等级 1
    • 细胞背景
     
    种属 人源
    组织来源
    肿瘤类型 星形细胞瘤
    供体信息 47岁,男性,白种人
    背景描述 J. Ponten及其同事在1966年至1969年间从恶性胶质瘤中提取的许多细胞系之一(参见U-87 MG, U-138 MG和U-373 MG)。
    • 遗传背景
     
    同工酶 Me-2, 1, PGM3, 2, PGM1, 2, ES-D, 1, AK-1, 1-2, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0001
    致瘤性 裸鼠可成瘤
    抗原表达 Blood Type A, Rh+, HLA Aw24, A28, B12, Bw47
    核型 The line has a near pentaploid chromosome number and a wide range of chromosome number distribution (40% of the cells had numbers ranging from 110 to 115). The following 14 markers were found in most metaphases: t(1p,2p), t(3p,?), t(4p,11q), t(7p,22q), M6, t(9q,?), i(11q)18q t(10q,?), M14, M15, M16, M17 and t(10q,22q), 6 of these were found in some and 10 were seen in one only. Normal chromosomes 7, 8, 12, 19, 20 and 22 had 5 to 6 copies per cell, the x had two copies and the Y was absent.
    STR鉴定
    STR位点信息
    STR鉴定图
    Amelogenin X,Y
    CSF1PO 11,12
    D1S1656 11
    D2S441 11,15
    D2S1338 23,25
    D3S1358 15
    D5S818 11
    D7S820 9
    D8S1179 14,15
    D10S1248 13
    D12S391 19,22
    D13S317 9 (ATCC=HTB-15; Cosmic-CLP=687588; Technion Genomics Center BCF) 9,11 (CLS=300362; PubMed=22570425; PubMed=27582061)
    D16S539 12,13
    D18S51 13
    D19S433 13
    D21S11 27,32.2
    D22S1045 17
    FGA 18,23 (PubMed=27582061) 23 (ATCC=HTB-15; CLS=300362; Technion Genomics Center BCF; PubMed=22570425)
    Penta D 10,13 (ATCC=HTB-15; Technion Genomics Center BCF; PubMed=22570425; PubMed=27582061) 13 (CLS=300362)
    Penta E 7 (ATCC=HTB-15; CLS=300362; Technion Genomics Center BCF; PubMed=22570425) 7,13 (PubMed=27582061)
    TH01 6
    TPOX 8
    vWA 18
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