- 细胞信息
发货规格 1×10⁶ 别名 U-118MG,U-118-MG,U118-MG,U118MG,u1118mg,118MG 形态 混合细胞样 生长特性 贴壁 培养条件 DMEM+10%FBS+1%P/S 冻存条件 90%FBS+10%DMSO 传代方式 胰酶消化 传代比例 1:3~1:6 换液频率 每周2~3次 生物安全等级 1
- 细胞背景
种属 人源 组织来源 脑 肿瘤类型 星形细胞瘤 供体信息 47岁,男性,白种人 背景描述 J. Ponten及其同事在1966年至1969年间从恶性胶质瘤中提取的许多细胞系之一(参见U-87 MG, U-138 MG和U-373 MG)。
- 遗传背景
同工酶 Me-2, 1, PGM3, 2, PGM1, 2, ES-D, 1, AK-1, 1-2, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0001 致瘤性 裸鼠可成瘤 抗原表达 Blood Type A, Rh+, HLA Aw24, A28, B12, Bw47 核型 The line has a near pentaploid chromosome number and a wide range of chromosome number distribution (40% of the cells had numbers ranging from 110 to 115). The following 14 markers were found in most metaphases: t(1p,2p), t(3p,?), t(4p,11q), t(7p,22q), M6, t(9q,?), i(11q)18q t(10q,?), M14, M15, M16, M17 and t(10q,22q), 6 of these were found in some and 10 were seen in one only. Normal chromosomes 7, 8, 12, 19, 20 and 22 had 5 to 6 copies per cell, the x had two copies and the Y was absent. STR鉴定STR位点信息STR鉴定图![]()
Amelogenin X,Y CSF1PO 11,12 D1S1656 11 D2S441 11,15 D2S1338 23,25 D3S1358 15 D5S818 11 D7S820 9 D8S1179 14,15 D10S1248 13 D12S391 19,22 D13S317 9 (ATCC=HTB-15; Cosmic-CLP=687588; Technion Genomics Center BCF) 9,11 (CLS=300362; PubMed=22570425; PubMed=27582061) D16S539 12,13 D18S51 13 D19S433 13 D21S11 27,32.2 D22S1045 17 FGA 18,23 (PubMed=27582061) 23 (ATCC=HTB-15; CLS=300362; Technion Genomics Center BCF; PubMed=22570425) Penta D 10,13 (ATCC=HTB-15; Technion Genomics Center BCF; PubMed=22570425; PubMed=27582061) 13 (CLS=300362) Penta E 7 (ATCC=HTB-15; CLS=300362; Technion Genomics Center BCF; PubMed=22570425) 7,13 (PubMed=27582061) TH01 6 TPOX 8 vWA 18