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- 规格：20μl质粒
- 质粒图谱1： png/1-1F92FZA4622.png
质粒序列： 点击查看序列LOCUS Exported 3618 bp ds-DNA circular SYN 22-SEP-2017DEFINITION synthetic circular DNAACCESSION .VERSION .KEYWORDS .SOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 3618)AUTHORS .TITLE Direct SubmissionJOURNAL Exported Sep 27, 2017 from MiaoLingPlasmidhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..3618/organism="synthetic DNA construct"/mol_type="other DNA"promoter 20..38/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"CDS 164..181/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"CDS 185..217/codon_start=1/product="leader peptide from bacteriophage T7 gene 10"/label=T7 tag (gene 10 leader)/note="promotes efficient translation in E. coli"/translation="MASMTGGQQMG"CDS 221..244/codon_start=1/product="Xpress(TM) epitope tag, including an enterokinaserecognition and cleavage site"/label=Xpress(TM) tag/translation="DLYDDDDK"CDS 251..952/codon_start=1/product="monomeric cyan-excitable red fluorescent protein(Laviv et al., 2016)"/label=mCyRFP1/translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTARIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGGVLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCDKKLKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARYSNLGGGMDELYK"terminator 1033..1080/label=T7 terminator/note="transcription terminator for bacteriophage T7 RNApolymerase"rep_origin 1177..1632/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 1658..1762/gene="bla"/label=AmpR promoterCDS 1763..2623/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"rep_origin 2794..3382/direction=RIGHT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"ORIGIN1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct61 agaatcaccg agtttattct tgacacctga tgcgatgaat gatataatag gaaagtactg121 ttttgtttaa ctttaagaag gggatataca tatgcggggt tctcatcatc atcatcatca181 tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga241 taaggatccg atggtgagca agggcgagga gctgatcaag gagaacatga gaagcaagct301 gtacctggaa ggcagcgtga acggccacca gttcaagtgc acccacgaag gggagggcaa361 gccctacgag ggcaagcaga ccgcgaggat caaggtggtg gagggaggcc ccctgccgtt421 cgcattcgac atcctggcca ccatgtttat gtacgggagc aaggtgttca tcaagtaccc481 cgccgacctc cccgattatt ttaagcagtc cttccctgag ggcttcacat gggagagagt541 catggtgttc gaagacgggg gcgtgctgac cgccacccag gacaccagcc tccaggacgg601 cgagctcatc tacaacgtca agctcagagg ggtgaacttc ccagccaacg gccccgtgat661 gcagaagaaa acactgggct gggagcccag caccgagacc atgtaccccg ctgacggcgg721 cctggaaggc agatgcgaca agaagctgaa gctcgtgggc gggggccacc tgcacgtcaa781 cttcaagacc acatacaagt ccaagaaacc cgtgaagatg cccggcgtcc actacgtgga841 ccgcagactg gaaagaatca aggaggccga caacgagacc tacgtcgagc agtacgagca901 cgctgtggcc agatactcca acctgggcgg aggcatggac gagctgtaca agtaatgaat961 tcgaagcttg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc1021 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg1081 ctgaaaggag gaactatatc cggatctggc gtaatagcga agaggcccgc accgatcgcc1141 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa1201 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc1261 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag1321 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca1381 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc1441 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa1501 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct1561 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa1621 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt1681 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca1741 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt1801 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga1861 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa1921 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct1981 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat2041 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga2101 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc2161 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat2221 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa2281 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac2341 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa2401 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc2461 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc2521 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag2581 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta2641 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa2701 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc2761 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat2821 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga2881 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt2941 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata3001 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac3061 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg3121 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg3181 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag3241 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct3301 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc3361 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt3421 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg3481 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga3541 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg3601 gccgattcat taatgcag//
