• 商品货号：ZT761

• 规格：10μl质粒
• 启动子：无
• 复制子：pVS1 oriV，pUC ori
• 终止子：NOS terminator
• 质粒大小：10658bp
• 原核抗性：卡那霉素Kan
• 筛选标记：潮霉素Hyg
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：         pCAMBIA1391是一个植物启动子检测报告载体，可通过MCS克隆进需要研究的启动子。           The pCambia vector backbone is derived from the pPZP vectors. The pCambia1391 vector offers:
                High copy number in E.coli for high DNA yields  pVS1 replicon for high stability in Agrobacterium
                Small size  Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
                Bacterial selection with kanamycin
                Plant selection with hygromycin B
                Simple means to construct translational fusions to gusA reporter genes
         Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins. Designed to utilize gusA as a true reporter of gene expression by fusion construction this vector contains a promoterless, non-intron gusA gene that retains the initiation codon of the NcoI site. This permits simple construction of carboxy-terminus protein fusions to gusA.
• 质粒图谱1： png/1-1G0230Z34A62.png

质粒序列： 点击查看序列

LOCUS       Exported               10658 bp ds-DNA     circular SYN 23-OCT-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 10658)

  AUTHORS   .

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..10658

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             20..1825

                     /codon_start=1

                     /gene="uidA (or gusA) from E. coli"

                     /product="beta-glucuronidase"

                     /label=GUS

                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA

                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM

                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF

                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV

                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL

                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA

                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK

                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL

                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT

                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP

                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"

     CDS             1832..1849

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /label=6xHis

                     /translation="HHHHHH"

     terminator      1881..2133

                     /label=NOS terminator

                     /note="nopaline synthase terminator and poly(A) signal"

     misc_feature    2155..2179

                     /label=RB T-DNA repeat

                     /note="right border repeat from nopaline C58 T-DNA"

     CDS             3479..4108

                     /codon_start=1

                     /product="stability protein from the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 StaA

                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR

                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP

                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI

                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

     CDS             4537..5610

                     /codon_start=1

                     /product="replication protein from the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 RepA

                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW

                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS

                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP

                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE

                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR

                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI

                     LVMRYRNLIEGEASAGS"

     rep_origin      5676..5870

                     /label=pVS1 oriV

                     /note="origin of replication for the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

     misc_feature    6214..6354

                     /label=bom

                     /note="basis of mobility region from pBR322"

     rep_origin      complement(6540..7128)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(7215..8009)

                     /codon_start=1

                     /gene="aphA-3"

                     /product="aminoglycoside phosphotransferase"

                     /label=KanR

                     /note="confers resistance to kanamycin"

                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM

                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED

                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT

                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA

                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"

     misc_feature    8434..8458

                     /label=LB T-DNA repeat

                     /note="left border repeat from nopaline C58 T-DNA"

     polyA_signal    8536..8710

                     /label=CaMV poly(A) signal

                     /note="cauliflower mosaic virus polyadenylation signal"

     CDS             complement(8750..9775)

                     /codon_start=1

                     /gene="aph(4)-Ia"

                     /product="aminoglycoside phosphotransferase from E. coli"

                     /label=HygR

                     /note="confers resistance to hygromycin"

                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG

                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP

                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ

                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF

                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG

                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK

                     K"

     promoter        complement(9843..10520)

                     /label=CaMV 35S promoter (enhanced)

                     /note="cauliflower mosaic virus 35S promoter with a 

                     duplicated enhancer region"

     misc_feature    10585..10644

                     /label=MCS

ORIGIN

        1 catggtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact

       61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg

      121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt

      181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat

      241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg

      301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga

      361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa

      421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa

      481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat

      541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca

      601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga

      661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca

      721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac

      781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc

      841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg

      901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca

      961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga

     1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt

     1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta

     1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct

     1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga

     1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact

     1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac

     1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag

     1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa

     1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc

     1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg

     1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc

     1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg

     1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga

     1801 aaaaccgcag cagggaggca aacaagctag ccaccaccac caccaccacg tgtgaattgg

     1861 tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga

     1921 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg

     1981 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc

     2041 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat

     2101 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag tgtttgacag

     2161 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa

     2221 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc

     2281 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga

     2341 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag

     2401 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag

     2461 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg