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- 规格：20μl质粒
- 启动子：无
- 复制子：pVS1 oriV，pUC ori
- 终止子：NOS terminator
- 质粒大小：10658bp
- 原核抗性：卡那霉素Kan
- 筛选标记：潮霉素Hyg
- 克隆菌株：大肠杆菌Stbl3
- 质粒简介： pCAMBIA1391是一个植物启动子检测报告载体，可通过MCS克隆进需要研究的启动子。 The pCambia vector backbone is derived from the pPZP vectors. The pCambia1391 vector offers:
High copy number in E.coli for high DNA yields pVS1 replicon for high stability in Agrobacterium
Small size Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
Plant selection with hygromycin B
Simple means to construct translational fusions to gusA reporter genes
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins. Designed to utilize gusA as a true reporter of gene expression by fusion construction this vector contains a promoterless, non-intron gusA gene that retains the initiation codon of the NcoI site. This permits simple construction of carboxy-terminus protein fusions to gusA.- 质粒图谱1： png/1-1G0230Z34A62.png
质粒序列： 点击查看序列
