• 规格：20μl质粒
• 原核抗性：壮观霉素Spe
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：              pMN20是一个植物超表达质粒，有CaMV 35S enhancers.
• 质粒图谱1： png/1-1G203125ZME.png

质粒序列： 点击查看序列

LOCUS       Exported               11073 bp ds-DNA     circular SYN 03-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..11073

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    complement(696..2051)

                     /label=4×35Se

     misc_feature    2388..2412

                     /label=RB T-DNA repeat

                     /note="right border repeat from nopaline C58 T-DNA"

     CDS             3712..4341

                     /codon_start=1

                     /product="stability protein from the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 StaA

                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR

                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP

                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI

                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

     CDS             4775..5842

                     /codon_start=1

                     /product="replication protein from the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 RepA

                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA

                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR

                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR

                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL

                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA

                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV

                     MRYRNLIEGEASAGS"

     rep_origin      5908..6102

                     /label=pVS1 oriV

                     /note="origin of replication for the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

     misc_feature    6446..6586

                     /label=bom

                     /note="basis of mobility region from pBR322"

     rep_origin      complement(6772..7360)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(7604..8395)

                     /codon_start=1

                     /gene="aadA"

                     /product="aminoglycoside adenylyltransferase (Murphy, 

                     1985)"

                     /label=Spe

                     /note="confers resistance to spectinomycin and 

                     streptomycin"

                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH

                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK

                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF

                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP

                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"

     misc_feature    8923..8947

                     /label=LB T-DNA repeat

                     /note="left border repeat from nopaline C58 T-DNA"

     polyA_signal    9045..9219

                     /label=CaMV poly(A) signal

                     /note="cauliflower mosaic virus polyadenylation signal"

     CDS             complement(9273..10070)

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR

                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL

                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH

                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI

                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     promoter        complement(10130..10808)

                     /label=CaMV 35S promoter (enhanced)

                     /note="cauliflower mosaic virus 35S promoter with a 

                     duplicated enhancer region"

ORIGIN

        1 ggtcgactct agaggatccc cgggtaccga gctcgagatc tagatatcga taagcttatc

       61 gataccgtcg acctcgaggg gccaacagag cctggcgttc cccttttgca ttgagaccga

      121 tgttcgttcc ggaaccttgc acgccccaga gcttctcacc gttcacgaca atttccttct

      181 cgttgaggtc ggtcgcgcca tgtcggatga aataaaaact tttgatacca gcgggggcct

      241 tcgcagagcc gaggtaggtc tgagaaattg gcattttcac gtgtggaaga tatgaatttt

      301 tttgagaaac tagataagat taatgaatat cggtgttttg gttttttctt gtggccgtct

      361 ttgtttatat tgagattttt caaatcagtg cgcaagacgt gacgtaagta tccgagtcag

      421 tttttatttt tctactaatt tggtcgttta tttcggcgtg taggacatgg caaccgggcc

      481 tgaatttcgc gggtattctg tttctattcc aactttttct tgatccgcag ccattaacga

      541 cttttgaata gatacgctga cacgccaagc ctcgctattc aaaagtgtac caaacaacgc

      601 tttacagcaa gaacggaatg cgcgtgacgc tcgcggtgac gccatttcgc cttttcagaa

      661 atggataaat agccttctca aacactgata gtttcggatc tagatatcac atcaatccac

      721 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgttcctcg tgggtggggg

      781 tccatctttg ggaccactgt cggtagaggc atcttgaacg atagcctttc ctttatcgca

      841 atgatggcat ttgtagaagc catcttcctt ttctactgtc ctttcgatga agtgacagat

      901 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat

      961 agccctctgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg

     1021 ctccaccatg ttggggatct agatatcaca tcaatccact tgctttgaag acgtggttgg

     1081 aacgtcttct ttttccacga tgttcctcgt gggtgggggt ccatctttgg gaccactgtc

     1141 ggtagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtagaagcc

     1201 atcttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat ggaatccgag

     1261 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctctggt cttctgagac

     1321 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggggatcta

     1381 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat

     1441 gttcctcgtg ggtgggggtc catctttggg accactgtcg gtagaggcat cttgaacgat

     1501 agcctttcct ttatcgcaat gatggcattt gtagaagcca tcttcctttt ctactgtcct

     1561 ttcgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct

     1621 ttgttgaaaa gtctcaatag ccctctggtc ttctgagact gtatctttga tattcttgga

     1681 gtagacgaga gtgtcgtgct ccaccatgtt ggggatctag atatcacatc aatccacttg

     1741 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ttcctcgtgg gtgggggtcc

     1801 atctttggga ccactgtcgg tagaggcatc ttgaacgata gcctttcctt tatcgcaatg

     1861 atggcatttg tagaagccat cttccttttc tactgtcctt tcgatgaagt gacagatagc

     1921 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc

     1981 cctctggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc

     2041 caccatgttg gggatccact agttctagag cggccgccac cgcggtggag ctcgaattcg

     2101 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac

     2161 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca

     2221 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat

     2281 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag cagcttgagc

     2341 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat

     2401 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt

     2461 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg

     2521 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag

     2581 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg

     2641 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt

     2701 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct

     2761 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg

     2821 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc

     2881 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc

     2941 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg

     3001 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg

     3061 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc

     3121 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca

     3181 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag

     3241 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg

     3301 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg

     3361 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca

     3421 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc

     3481 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct

     3541 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac

     3601 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt

     3661 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt

     3721 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc

     3781 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt

     3841 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc

     3901 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga

     3961 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt

     4021 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag

     4081 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc

     4141 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt

     4201 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc

     4261 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa

     4321 a