• 规格：20μl质粒
• 原核抗性：氨苄青霉素
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：           pUC35s-mCherry植物红色荧光瞬转表达质粒，
• 质粒图谱1： png/1-1G201133A93P.png

质粒序列： 点击查看序列

LOCUS       Exported                4053 bp ds-DNA     circular SYN 01-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..4053

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        439..784

                     /label=CaMV 35S promoter

                     /note="strong constitutive promoter from cauliflower mosaic

                     virus"

     CDS             823..1533

                     /codon_start=1

                     /product="monomeric derivative of DsRed fluorescent protein

                     (Shaner et al., 2004)"

                     /label=mCherry

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG

                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF

                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK

                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA

                     EGRHSTGGMDELYK"

     terminator      1550..1802

                     /label=NOS terminator

                     /note="nopaline synthase terminator and poly(A) signal"

     rep_origin      complement(2234..2822)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(2993..3853)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(3854..3958)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttcaaag atacagtctc

      421 agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg

      481 attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc

      541 ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag

      601 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac

      661 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca

      721 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag

      781 aacacggggg actctagagg atccccgggt ggtcagtccc ttatggtgag caagggcgag

      841 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc

      901 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc

      961 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg

     1021 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac

     1081 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac

     1141 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag

     1201 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg

     1261 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc

     1321 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac

     1381 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc

     1441 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac

     1501 tccaccggcg gcatggacga gctgtacaag tgagagctcg aatttccccg atcgttcaaa

     1561 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat

     1621 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt

     1681 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa

     1741 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga

     1801 tcggggtacc gagctcgaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt

     1861 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg

     1921 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg

     1981 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc

     2041 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc

     2101 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata

     2161 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg

     2221 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct

     2281 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa

     2341 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc

     2401 tcccttcggg aagcgtggcg ctttctcaaa gctcacgctg taggtatctc agttcggtgt

     2461 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg

     2521 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg

     2581 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct

     2641 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc

     2701 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg

     2761 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc

     2821 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt

     2881 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa

     2941 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat

     3001 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct

     3061 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg

     3121 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag

     3181 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta

     3241 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg

     3301 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg

     3361 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct

     3421 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta

     3481 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg

     3541 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc

     3601 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg

     3661 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga

     3721 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg

     3781 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat

     3841 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc

     3901 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca

     3961 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct

     4021 ataaaaatag gcgtatcacg aggccctttc gtc

//