• 规格：20μl质粒
• 启动子：PH promoter
• 复制子：pUC
• 原核抗性：氨苄青霉素Amp
• 筛选标记：庆大霉素Gen
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：          pFastBac1-Gus是一个昆虫细胞杆状病毒胞内表达质粒。
• 质粒图谱1： png/1-1G221150116463.png

质粒序列： 点击查看序列

LOCUS       Exported                6661 bp ds-DNA     circular SYN 13-OCT-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..6661

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     rep_origin      2..457

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        484..588

                     /gene="bla"

                     /label=AmpR promoter

     CDS             589..1449

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      1620..2208

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     mobile_element  2511..2735

                     /mobile_element_type="transposon:Tn7"

                     /note="mini-Tn7 element (right end of the Tn7 transposon)"

     CDS             complement(2802..3335)

                     /codon_start=1

                     /gene="aacC1"

                     /product="gentamycin acetyltransferase"

                     /label=GmR

                     /note="confers resistance to gentamycin"

                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD

                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS

                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR

                     EEVMHFDIDPSTAT"

     promoter        complement(3524..3552)

                     /gene="intI1 (promoter lies within the coding sequence)"

                     /label=Pc promoter

                     /note="class 1 integron promoter"

     promoter        3905..3996

                     /gene="polh from Autographa californica"

                     /label=polyhedrin promoter

                     /note="promoter for the baculovirus polyhedrin gene"

     CDS             4081..5892

                     /codon_start=1

                     /gene="uidA (or gusA) from E. coli"

                     /product="beta-glucuronidase"

                     /label=GUS

                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI

                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV

                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD

                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ

                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF

                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW

                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD

                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD

                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY

                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK

                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"

     polyA_signal    6152..6286

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     mobile_element  6315..6480

                     /mobile_element_type="transposon:Tn7"

                     /note="mini-Tn7 element (left end of the Tn7 transposon)"

ORIGIN

        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc

       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc

      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt

      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg

      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt

      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta

      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt

      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat

      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg

      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa

      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac

      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac

      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt

      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc

      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca

      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc

      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag

     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa

     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg

     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa

     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg

     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt

     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt

     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag

     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat

     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct

     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct

     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca

     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc

     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc

     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct

     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag

     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc

     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg

     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag

     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt

     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac

     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg

     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc

     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg

     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct

     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga

     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag

     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt

     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga

     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac

     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg

     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg

     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca

     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact

     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc

     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta

     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct

     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg

     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg

     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca

     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa

     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa

     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca

     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac

     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc

     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg

     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt

     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt

     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa

     3901 taggatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt

     3961 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc

     4021 accatcgggc gcggatccat atatagggcc cgggttataa ttacctcagg tcgacgtccc

     4081 atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca

     4141 ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa

     4201 gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt

     4261 cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca

     4321 ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat

     4381 aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg

     4441 tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg

     4501 cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac

     4561 ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg