- 商品货号:FH1714
- 规格:10μl质粒
- 原核抗性:卡那霉素Kan
- 筛选标记:遗传霉素G418
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: ph7SK-gRNA是一个哺乳细胞gRNA表达质粒。
- 质粒图谱1: png/1-1G220163536209.png
质粒序列: 点击查看序列LOCUS Exported 3504 bp ds-DNA circular SYN 20-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..3504/organism="synthetic DNA construct"/mol_type="other DNA"rep_origin complement(3..458)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"misc_feature 691..933/label=7SK promotermisc_RNA 952..1027/label=gRNA scaffold/note="guide RNA scaffold for the Streptococcus pyogenesCRISPR/Cas9 system"rep_origin complement(1524..2112)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(2189..2983)/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=NeoR/KanR/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"promoter complement(3018..3375)/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 3032..3167/label=SV40 ori/note="SV40 origin of replication"promoter complement(3377..3481)/gene="bla"/label=AmpR promoterORIGIN1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctt661 taattctagt actatgcatc gtctcattgt ctgcagtatt tagcatgccc cacccatctg721 caaggcattc tggatagtgt caaaacagcc ggaaatcaag tccgtttatc tcaaacttta781 gcattttggg aataaatgat atttgctatg ctggttaaat tagattttag ttaaatttcc841 tgctgaagct ctagtacgat aagcaacttg acctaagtgt aaagttgaga cttccttcag901 gtttatatag cttgtgcgcc gcttgggtac ctcgggtctt cgagaagacc tgttttagag961 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag1021 tcggtgcttt ttttctcgga ctgatgacct gagacgccgc ggtggagctc cagcttttgt1081 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg1141 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa1201 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct1261 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga1321 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc1381 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa1441 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt1501 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa1561 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt1621 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg1681 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc1741 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc1801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta1861 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct1921 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc1981 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa2041 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa2101 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa2161 aactcacgtt aagggatttt ggtcatgatc agaagaactc gtcaagaagg cgatagaagg2221 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt2281 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg2341 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat2401 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct2461 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct2521 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt2581 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga2641 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc2701 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa2761 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac2821 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg2881 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc2941 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc3001 ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc3061 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag3121 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg3181 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg3241 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt3301 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg3361 ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat cagggttatt3421 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc3481 gcacatttcc ccgaaaagtg ccac//
服务时间:24小时在线客服
客服热线:400-000-7085
丰晖生物
中国优质生物资源平台