• 规格：20μl质粒
• 启动子：35S
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：        pUC-SPYCE(MR)是一个植物双分子荧光BIFC质粒。
• 质粒图谱1： png/1-1P104134043227.png

质粒序列： 点击查看序列

LOCUS       Exported                4113 bp ds-DNA     circular SYN 04-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..4113

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        863..1208

                     /label=CaMV 35S promoter

                     /note="strong constitutive promoter from cauliflower mosaic

                     virus"

     CDS             1226..1477

                     /codon_start=1

                     /product="C-terminal fragment of mVenus for use in 

                     bimolecular fluorescence complementation (BiFC) (Kodama and

                     Hu, 2010)"

                     /label=VC155

                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH

                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"

     CDS             1478..1504

                     /codon_start=1

                     /product="HA (human influenza hemagglutinin) epitope tag"

                     /label=HA

                     /translation="YPYDVPDYA"

     misc_feature    1505..1564

                     /label=MCS

     terminator      1575..1827

                     /label=NOS terminator

                     /note="nopaline synthase terminator and poly(A) signal"

     promoter        2326..2430

                     /gene="bla"

                     /label=AmpR promoter

     CDS             2431..3291

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      3462..4050

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat

       61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag

      121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac

      181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc

      241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt

      301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca

      361 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg

      421 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg

      481 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca

      541 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga

      601 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag

      661 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg

      721 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa

      781 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag

      841 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc

      901 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg

      961 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg

     1021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc

     1081 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg

     1141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg

     1201 agagaacacg ggggactcta gaatggacaa gcagaagaac ggcatcaagg tgaacttcaa

     1261 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac

     1321 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa

     1381 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc

     1441 cgccgggatc actctcggca tggacgagct gtacaagtac ccatacgatg ttccagatta

     1501 cgctgggccc aggcctacta gtggatccgt cgacctcgag ggtaccgctc ccgggtagga

     1561 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg

     1621 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa

     1681 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat

     1741 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc

     1801 gcgcggtgtc atctatgtta ctagatcggg aattcactgg ccgtcgtttt acaacgtcgt

     1861 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc

     1921 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg

     1981 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac

     2041 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga

     2101 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac

     2161 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg

     2221 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata

     2281 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt

     2341 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa

     2401 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt

     2461 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa

     2521 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac

     2581 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt

     2641 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt

     2701 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat

     2761 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac

     2821 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg

     2881 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc

     2941 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa

     3001 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag

     3061 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct

     3121 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat

     3181 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa

     3241 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac

     3301 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc

     3361 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc

     3421 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg

     3481 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg

     3541 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca

     3601 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg

     3661 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg

     3721 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga

     3781 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac

     3841 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat

     3901 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc

     3961 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga

     4021 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc

     4081 ctggcctttt gctggccttt tgctcacatg ttc

//