• 规格：20μl质粒
• 启动子：2X35S promoter + TL enhancer
• 复制子：pUC ori
• 终止子：35S terminator
• 质粒分类：植物系列质粒；植物报告质粒；双分荧光质粒
• 质粒大小：4417bp
• 质粒标签：C-n(1-174)EYFP
• 原核抗性：氨苄青霉素Amp
• 筛选标记：无
• 克隆菌株：DH5α等大肠杆菌
• 表达宿主：植物
• 诱导方式：无需诱导，组成型表达
• 5测序引物：35S：GACGCACAATCCCACTATCC
• 3测序引物：35S-ter：CTCAACACATGAGCGAAACCC
• 质粒简介：           pSAT6-nEYFP-N1质粒是一个用于植物双分子荧光实验的载体，2X35S promoter + TL enhancer驱动EYFP的表达。
• 质粒图谱1： png/1-1FR510592TB.png

质粒序列： 点击查看序列

LOCUS       Exported                4417 bp ds-DNA     circular SYN 25-AUG-2017

DEFINITION  synthetic circular DNA

KEYWORDS    pSAT6-nEYFP-N1

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4417)

  AUTHORS   NCBI Entrez

  TITLE     Direct Submission

  JOURNAL   Exported Aug 25, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..4417

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    complement(149..398)

                     /label=ALPHA

                     /note="/vntifkey=21"

     misc_feature    complement(399..428)

                     /label=PI-PspI

                     /note="/vntifkey=21"

     misc_feature    429..1320

                     /label=2X35S promoter + TL enhancer

                     /label=2X35Spromoter+TLenhancer

                     /label=nonstandard type: misc_marker

                     /note="/vntifkey=22"

     primer_bind     1110..1129

                     /label=35S

     misc_feature    1323..1394

                     /label=MCS

                     /note="/vntifkey=21"

     misc_feature    1395..1919

                     /label=n(1-174)EYFP

                     /note="/vntifkey=21"

     misc_feature    1938..2156

                     /label=35S terminator

                     /label=35S erminator

                     /note="/vntifkey=21"

     primer_bind     complement(2041..2061)

                     /label=35S-ter

     misc_feature    2157..2186

                     /label=PI-PspI

                     /note="/vntifkey=21"

     misc_feature    complement(2598..3186)

                     /label=pUC ori

     misc_feature    complement(3360..4217)

                     /label=Amp

                     /label=AMP(R)

                     /note="/vntifkey=21"

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct

      421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag

      481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa

      541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg

      601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct

      661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag

      721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca

      781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga

      841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc

      901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata

      961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac

     1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt

     1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc

     1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa

     1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta

     1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag

     1321 ccatggtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg

     1381 ggcccgggat cctgatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc

     1441 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg

     1501 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg

     1561 tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc

     1621 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg

     1681 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg

     1741 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca

     1801 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg

     1861 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggactgaa

     1921 gatccaccta gtctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct

     1981 ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata

     2041 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa

     2101 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgacgc ggccgcaccc

     2161 ataataccca taatagctgt ttgccagtaa tcatggtcat agctgtttcc tgtgtgaaat

     2221 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg

     2281 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag

     2341 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt

     2401 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg

     2461 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg

     2521 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag

     2581 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga

     2641 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct

     2701 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc

     2761 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg

     2821 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc

     2881 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca

     2941 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag

     3001 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct

     3061 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc

     3121 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga

     3181 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca

     3241 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat

     3301 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac

     3361 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt

     3421 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt

     3481 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag

     3541 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct

     3601 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt

     3661 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc

     3721 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt

     3781 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg

     3841 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg

     3901 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct

     3961 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc

     4021 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt

     4081 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt

     4141 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg

     4201 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat

     4261 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg

     4321 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta

     4381 acctataaaa ataggcgtat cacgaggccc tttcgtc

//