• 规格：20μl质粒
• 质粒图谱1： png/1-1F92614403XO.png

质粒序列： 点击查看序列

LOCUS       Exported                4402 bp ds-DNA     circular SYN 26-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4402)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 26, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..4402

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    435..1319

                     /label=2X35S promoter + TL enhancer

     CDS             1323..1841

                     /codon_start=1

                     /product="N-terminal fragment of mVenus for use in 

                     bimolecular fluorescence complementation (BiFC) (Kodama and

                     Hu, 2010)"

                     /label=n(1-174)EYFP

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIE"

     misc_feature    1845..1910

                     /label=MCS

                     /note="multiple cloning site"

     misc_feature    1923..2133

                     /label=35S terminator

     rep_origin      complement(2583..3171)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3342..4202)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(4203..4307)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct

      421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag

      481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa

      541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg

      601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct

      661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag

      721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca

      781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga

      841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc

      901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata

      961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac

     1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt

     1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc

     1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa

     1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta

     1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag

     1321 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg

     1381 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct

     1441 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca

     1501 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga

     1561 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct

     1621 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc

     1681 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc

     1741 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga

     1801 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggactccgga ctcagatctc

     1861 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc acctagtcta

     1921 gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga

     1981 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt

     2041 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa

     2101 aatttctaat tcctaaaacc aaaatccagt gacgcggccg cacccataat acccataata

     2161 gctgtttgcc agtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca

     2221 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg

     2281 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg

     2341 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc

     2401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta

     2461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag

     2521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg

     2581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg

     2641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg

     2701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga

     2761 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc

     2821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt

     2881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact

     2941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg

     3001 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt

     3061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt

     3121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct

     3181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg

     3241 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt

     3301 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt

     3361 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc

     3421 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg

     3481 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc

     3541 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg

     3601 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca

     3661 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga

     3721 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct

     3781 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg

     3841 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca

     3901 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata

     3961 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct

     4021 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact

     4081 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa

     4141 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc

     4201 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga

     4261 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga

     4321 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg

     4381 cgtatcacga ggccctttcg tc

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