• 规格：20μl质粒
• 质粒图谱1： png/1-1F92615024K15.png

质粒序列： 点击查看序列

LOCUS       Exported                4031 bp ds-DNA     circular SYN 26-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4031)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 26, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..4031

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    413..1158

                     /label=3×CaMV 35S promoter

     misc_feature    1299..1355

                     /label=MCS

                     /note="multiple cloning site"

     misc_feature    1356..1567

                     /label=cEYFP

     misc_feature    1574..1784

                     /label=CaMV 35S terminator

     rep_origin      complement(2212..2800)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(2971..3831)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(3832..3936)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgg cgcgccaccg gtcaacatgt

      421 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag

      481 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc

      541 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca

      601 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga

      661 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa

      721 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat

      781 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc

      841 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga

      901 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga

      961 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa

     1021 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt

     1081 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc

     1141 atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct

     1201 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt taaagcaaaa

     1261 gcaattttct gaaaattttc accatttacg aacgatagag atctcgagct caagcttcga

     1321 attctgcagt cgacggtacc gcgggcccgg gatcctgggc agcgtgcagc tcgccgacca

     1381 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct

     1441 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct

     1501 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtgaagatc

     1561 cacctagtct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat

     1621 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt

     1681 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact

     1741 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacgcggcc gcggcgcgcc

     1801 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa

     1861 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac

     1921 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca

     1981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc

     2041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc

     2101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc

     2161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag

     2221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc

     2281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt

     2341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct

     2401 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg

     2461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct

     2521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat

     2581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg

     2641 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa

     2701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt

     2761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc

     2821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt

     2881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta

     2941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat

     3001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac

     3061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg

     3121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag

     3181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt

     3241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt

     3301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt

     3361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt

     3421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct

     3481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt

     3541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac

     3601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa

     3661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa

     3721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca

     3781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct

     3841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga

     3901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc

     3961 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag

     4021 gccctttcgt c

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