• 规格：20μl质粒
• 质粒图谱1： png/1-1F926111450257.png

质粒序列： 点击查看序列

LOCUS       Exported                4081 bp ds-DNA     circular SYN 26-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4081)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 26, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..4081

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    435..1322

                     /label=2X35S promoter + TL enhancer

     misc_feature    1323..1523

                     /label=nEYFP

     misc_feature    1524..1589

                     /label=MCS

                     /note="multiple cloning site"

     misc_feature    1602..1812

                     /label=35S terminator

     rep_origin      complement(2262..2850)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3021..3881)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(3882..3986)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct

      421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag

      481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa

      541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg

      601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct

      661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag

      721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca

      781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga

      841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc

      901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata

      961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac

     1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt

     1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc

     1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa

     1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta

     1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag

     1321 ccatgggcgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg

     1381 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc

     1441 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc

     1501 tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattctg

     1561 cagtcgacgg taccgcgggc ccgggatcca cctgatctag agtccgcaaa aatcaccagt

     1621 ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca

     1681 gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct

     1741 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca

     1801 aaatccagtg acgcggccgc acccataata cccataatag ctgtttgcca gtaatcatgg

     1861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc

     1921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg

     1981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc

     2041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact

     2101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta

     2161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag

     2221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc

     2281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta

     2341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg

     2401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc

     2461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac

     2521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac

     2581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg

     2641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga

     2701 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt

     2761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag

     2821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct

     2881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg

     2941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat

     3001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc

     3061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg

     3121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct

     3181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca

     3241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg

     3301 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg

     3361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc

     3421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag

     3481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg

     3541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag

     3601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat

     3661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg

     3721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca

     3781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca

     3841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat

     3901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag

     3961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa

     4021 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt

     4081 c

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