• 规格：20μl质粒
• 启动子：CaMV 35S promoter (enhanced)
• 复制子：pUC ori，pVS1 oriV
• 终止子：CaMV poly(A) signal
• 质粒分类：植物系列质粒；植物表达质粒；其它系列质粒
• 质粒大小：10531bp
• 原核抗性：卡那霉素Kan
• 筛选标记：潮霉素Hyg
• 克隆菌株：DH5α等大肠杆菌
• 表达宿主：植物
• 质粒简介：              pLSLR是一个植物的T-DNA质粒，拥有潮霉素筛选标记。Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.
• 质粒图谱1： png/1-1F91G62UOX.png

质粒序列： 点击查看序列

LOCUS       Exported               10531 bp ds-DNA     circular SYN 17-SEP-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 10531)

  TITLE     Direct Submission

  JOURNAL   Exported Sep 17, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..10531

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     rep_origin      1129..1323

                     /label=pVS1 oriV

                     /note="origin of replication for the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

     misc_feature    1667..1807

                     /label=bom

                     /note="basis of mobility region from pBR322"

     rep_origin      complement(1993..2581)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(2668..3462)

                     /codon_start=1

                     /gene="aphA-3"

                     /product="aminoglycoside phosphotransferase"

                     /label=KanR

                     /note="confers resistance to kanamycin"

                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM

                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED

                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT

                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA

                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"

     misc_feature    3887..3911

                     /label=LB T-DNA repeat

                     /note="left border repeat from nopaline C58 T-DNA"

     polyA_signal    3989..4163

                     /label=CaMV poly(A) signal

                     /note="cauliflower mosaic virus polyadenylation signal"

     CDS             complement(4189..5331)

                     /codon_start=1

                     /label=Hyg

                     /translation="MTHNPTILRKTFLYIRKFISFGEDTLKSPVSLYKSISPRMKKPEL

                     TATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYR

                     HFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAA

                     ADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELML

                     WAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACM

                     EQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA

                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPGSWR"

     promoter        complement(5253..5930)

                     /label=CaMV 35S promoter (enhanced)

                     /note="cauliflower mosaic virus 35S promoter with a 

                     duplicated enhancer region"

     misc_feature    8140..8164

                     /label=RB T-DNA repeat

                     /note="right border repeat from nopaline C58 T-DNA"

     CDS             9464..10093

                     /codon_start=1

                     /product="stability protein from the Pseudomonas plasmid 

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 StaA

                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR

                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP

                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI

                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

ORIGIN

        1 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt

       61 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc

      121 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc

      181 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct

      241 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc

      301 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga

      361 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc

      421 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg

      481 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg

      541 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat

      601 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt

      661 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga

      721 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt

      781 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg

      841 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg

      901 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga

      961 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg

     1021 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct

     1081 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac

     1141 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa

     1201 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga

     1261 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc

     1321 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct

     1381 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat

     1441 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga

     1501 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc

     1561 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca

     1621 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc

     1681 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt

     1741 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg

     1801 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg

     1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa

     1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc

     1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc

     2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag

     2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct

     2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta

     2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc

     2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc

     2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt

     2401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct

     2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc

     2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca

     2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta

     2641 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt

     2701 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc

     2761 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc

     2821 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt

     2881 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa

     2941 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc

     3001 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct

     3061 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata

     3121 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc

     3181 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt

     3241 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata

     3301 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac

     3361 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc

     3421 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct

     3481 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca

     3541 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca

     3601 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca

     3661 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca

     3721 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc

     3781 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg

     3841 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt

     3901 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt

     3961 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta

     4021 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc

     4081 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact

     4141 actcacacat tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga

     4201 tcccggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc

     4261 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga

     4321 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac

     4381 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga

     4441 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa

     4501 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc

     4561 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa

     4621 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc

     4681 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac

     4741 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg

     4801 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat

     4861 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt

     4921 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc

     4981 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt

     5041 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa

     5101 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa

     5161 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg

     5221 agag