细胞信息
发货规格	1×10⁶
别名	CaLu-3， CALU-3， Calu 3， Calu3， CALU3
形态	上皮细胞样
生长特性	贴壁
培养条件	MEM+10%FBS+1%P/S
冻存条件	90%FBS+10%DMSO
传代方式	胰酶消化
传代比例	1:2~1:4
换液频率	每周2~3次
生物安全等级	1
细胞背景
种属	人源
组织来源	肺
肿瘤类型	肺腺癌
供体信息	25岁白种人男性
背景描述	Calu-3细胞是1975年从一名25岁患者的肺腺癌中获得的人上皮细胞系。这些细胞表现出上皮形态，其特征是能够形成紧密连接、桥粒和微绒毛，反映了肺上皮的结构特征。
遗传背景
蛋白表达	Blood Type A， Rh
同工酶	AK-1， 1ES-D， 1G6PD， BGLO-I， 1-2Me-2， 1PGM1， 1PGM3， 1-2
基因表达	blood type A; Rh+
致瘤性	裸鼠
抗原表达	Blood Type A; Rh+
突变信息	Pleural effusion
核型	The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%. Approximately 20 markers were common to most S metaphases， of which i (1p)， t(12;?) and t(18;?) were generally paired; t(6t?) had an HSR segment of q arm， and M7 had two secondary constrictions. Normal chromosomes 1， 13， 15 and 17 were absent， and the X was disomic. No Y chromosome was detected in the QM stained preparations. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.

STR鉴定

STR位点信息

STR鉴定图

Amelogenin	X
CSF1PO	11,12
D2S1338	19
D3S1358	15,18
D5S818	11
D7S820	10,11
D8S1179	11,15
D13S317	12
D16S539	12,14
D18S51	14,17
D19S433	12,13
D21S11	28,30
FGA	25
Penta D	9,16
Penta E	5,21
TH01	6,9.3
TPOX	8
vWA	16,17