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| 发货规格 |
1×10⁶ |
| 别名 |
MRC5; MRC 5; MRCV; MRC-V; Medical Research Council cell strain-5 |
| 形态 |
成纤维细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
MEM+10%FBS+1%NEAA+1%丙酮酸钠+1%谷氨酰胺+1%双抗 |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
36~48h |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
| 培养注意事项 |
细胞在衰老前能够进行 42 至 46 次群体倍增。 |
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| 种属 |
人源 |
| 组织来源 |
肺 |
| 供体信息 |
妊娠14周白人男性胚胎 |
| 背景描述 |
MRC-5 是用于生产疫苗（包括甲型肝炎、脊髓灰质炎和 MCR-5）的二倍体细胞系，由从 J.P. Jacobs 于 1966 年从 14 周龄的白人男性胚胎的肺组织中分离出的成纤维细胞组成。这些细胞能够进行 42 到 46 个群体倍增。 |
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| 同工酶 |
G6PD， B |
| 病毒易感性 |
Human poliovirus 1；Herpes simplex virus；Vesicular stomatitis， Glasgow (Indiana)；Vesicular stomatitis， Orsay (Indiana) |
| 核型 |
Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46，XY karyotype. The modal chromosome number was 46， occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
