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| 发货规格 |
1×10⁶ |
| 别名 |
SW-620; SW 620; SW.620 |
| 形态 |
上皮细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
RPMI-1640+10%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
30~37h |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
人源 |
| 组织来源 |
大肠 |
| 肿瘤类型 |
结直肠腺癌 |
| 供体信息 |
51岁白人男性 |
| 背景描述 |
SW-620细胞系来自一名患有Dukes-C型结直肠癌的51岁男性的大肠，是结直肠癌的关键研究模型，特别是用于癌症生物标志物、化疗和转移性癌细胞的研究。SW-620细胞是研究细胞凋亡和抗失巢凋亡机制的关键，失巢凋亡是一种对防止转移至关重要的程序性细胞死亡形式。利用SW-620结肠癌细胞的研究已经深入到蛋白质组分析中，以了解不同条件下（如缺氧）的蛋白质组变化。低氧SW620细胞表现出特定的蛋白质组适应，这有助于化疗耐药性。 |
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| 蛋白表达 |
keratin |
| 同工酶 |
ES-D， 1；G6PD， B；PEP-D， 1；PGD， A；PGM1， 2；PGM3， 1 |
| 基因表达 |
carcinoembryonic antigen (CEA) 0.15 ng/106 cells/10 days; transforming growth factor alpha; matrilysin; CSAp negative; and colon antigen 3 negative; keratin positive by immunoperoxidase staining; c-myc; K-ras; H-ras; N-ras; Myb; sis; fos |
| 致癌基因 |
myc+; myb+; ras+; fos+; sis+; p53+; abl-; ros-; src- |
| 致瘤性 |
Yes， in nude mice。(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| 抗原表达 |
Blood type A; Rh+ |
| 突变信息 |
There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.The line is positive for expression of c-myc， K-ras， H-ras， N-ras， Myb， sis and fos oncogenes. |
| 核型 |
modal number = 50; range = 45 to 53. The stemline chromosome number is hyperdiploid with the 2S component occurring at 12% and 11 marker chromosomes were common to S metaphases. One of the 11 markers [7 (7p; 11p)] was disomic.， M1 was probably an HSR but the origin of the chromosome could not be identified. |
