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发货规格 |
1×10⁶ |
别名 |
HT 29; HT29 |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
RPMI1640+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
24h |
传代比例 |
1:2~1:4 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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种属 |
人源 |
组织来源 |
结肠 |
肿瘤类型 |
结肠腺癌 |
供体信息 |
44岁白人女性 |
背景描述 |
HT-29细胞系来源于II级人结直肠腺癌,是人类结肠癌研究的基石研究模型。HT22细胞来源于1964年一名44岁女性的原发性肿瘤,有助于促进我们对癌细胞粘附或侵袭机制的理解。作为一种人腺癌细胞系,HT-29细胞表现出与肠细胞等成熟肠细胞非常相似的特征,强调了它们在探索食物消化和营养生物利用度动态方面的效用。 |
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蛋白表达 |
CEA negative, p53 positive |
受体表达 |
urokinase receptor(u-PAR), vitamin D (moderate expression), no detectable plasminogen activator activity. |
同工酶 |
Me-2, 1, PGM3, 1-2, PGM1, 1-2, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0230 |
致癌基因 |
myc+, ras+, myb+, fos+, sis+, p53+, abl -, ros -, src |
致瘤性 |
Yes, in nude mice |
抗原表达 |
Blood Type A, Rh+, HLA A1, A3, B12, B17, Cw5, CD4 -, cell surface expression of galactose ceramide (a possible alternative receptor for HIV) |
病毒易感性 |
human immunodeficiency virus (HIV, LAV) |
核型 |
The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-,?) with a deleted short arm), t(7q,?), t(10q,?), i(13q), 19q+a. M6, ?t(8q,9q-), ?xp, M9, 6q+, t(13,?)a, t(13,?)b, 19q+b, M14, M15, 15p+, and xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis. |